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KMID : 0616120180490010047
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Korean Journal of Pharmacognosy
2018 Volume.49 No. 1 p.47 ~ p.54
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Quantitative Analysis of Bergenin in Astilbe rubra and Growth Inhibition Effect of Ethanol Extract on Hela Cells
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Kim A-Hyeon
Nho Jong-Hyun
Woo Kyeong-Wan
Gang Ju-Eun
Sim Mi-Ok
Kim Min-Suk
Ham Seong-Ho
Cho Hyun-Woo
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Abstract
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Astilbe rubra (AR) is a perennial, belongs to the Saxifragaceae family, it contains tannin and triterpene. AR has been used in republic korea to improve toxication, fever, pain and convulsion. Recently, number of natural products have been analyzed for potential pharmacological activities including anti-cancer, anti-obesity and anti-diabetic medication. Consequently, we investigated the growth inhibition effect of Astilbe rubra water extract (WAC), ethanol extract (EAC) and bergenin on Hela cell (human adenocarcinoma cell). From whole plant of A. rubra, bergenin was isolated by column chromatography and its structures were identified by 1H, 13 C NMR and IT TOF-ESI MS. High extraction efficiency of bergenin was shown at 0.95% under 60 min reflux extraction with 50% MeOH. The MTS assay showed that EAC (ethanol extract) treatment increased cell death in a dose-dependent manner. Moreover, EAC treatment on Hela cell increased apoptotic cell death and caspase-3 activity. Results suggest that EAC has growth inhibition effect on Hela cells, but not WAC and bergenin. 500 μg/mL EAC treatment inhibited Hela cell at 60.2±1.5%.
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KEYWORD
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Saxifragaceae, Astilbe rubra, Bergenin, Uterine cervical cancer
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